Fig. 6.

METH activates eNOS in BMVECs. (A) Primary BMVECs were either left untreated (NT) or treated with METH (1 μM) for the indicated times. Total protein extracts (ca. 50 μg) were analysed by immunoblotting with anti-phospho-S1177 eNOS antibodies. The blot was subsequently stripped and probed for total eNOS. (B) As in A, except that GPNT BMVECs were used. Densitometric quantification (normalised means ± SEM) of three independent experiments is shown on the bottom of each panel. *P < 0.05, **P < 0.01 (Dunnett's post test vs NT).