Fig. 7.

Effect of higher METH concentrations in BMVECs. (A) GPNT ECs were either left untreated (NT) or treated with METH during 1 h with the indicated METH concentrations. eNOS phosphorylation was quantified by immunoblotting as described in Fig. 5. (**P < 0.01, Dunnett's post test). (B, C) Changes in flux of 4 kDa FITC-dextran (B) or 70 kDa RITC-dextran (C) across primary BMVECs in response to 50 μM METH was measured as described in Fig. 1. (D) TEER changes were measured in monolayers of primary BMVECs in response to 50 μM METH as described in Fig. 1. (E) GPNT ECs were either left untreated or treated with METH (50 μM, 30 min) before lymphocyte adhesion (white) and migration (black) was determined as described in Fig. 5.