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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: J Am Chem Soc. 2012 Nov 8;134(46):18883–18885. doi: 10.1021/ja307179q

Figure 3.

Figure 3

Stopped-flow time course for the reaction of (~8 μM) bis-(pyren-1-yl)-DHFR with 1.25 μM NADPH and 100 μM dihydrofolate at pH 7.0. The reaction was initiated by rapid mixing with 0.2 mM dihydrofolate. Excitation was at 360 nm with a 450 nm cutoff filter for emission. The data (red trace) were fitted to a single-exponential curve (black curve).