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Increased levels of YggX protein in yggX* mutant. Western blot analysis was performed according to Harlow and Lane (59). Proteins were visualized by using alkaline phosphatase conjugated to anti-rabbit secondary antibody (Promega). Lanes A–C were loaded with crude cell-free extracts (1 μg protein) from strains DM5104, DM5105 (yggX*), and DM5647 (yggX∷Gm), respectively. Lane D was loaded with 1 ng purified YggX.
