Table A1.
Primers and probes used to amplify genes evaluated with qPCR.
| Gene | Ensembl accesion number | Primer foward | Primer reverse | Probe |
|---|---|---|---|---|
| EPS15 | ENSSSCT00000004284 | AGCGAGGTTCAGGATCTTCAAG | GGGCCTTCTGCTCATCCA | CCCAGAAACAGCAAGTACAGGAACTCCTTG |
| EZR | ENSSSCT00000004487 | GCAGCGGCAGCTGATGA | GTCGTTGTGGGTCCTCTTGTTC | ACCAACGAGCTGTCCCAGGCCA |
| GRLF1 | ENSSSCT00000003446 | CCCATACAACATGCAGATGGAT | ACCTCCTTTAGGGCATGTAGCTT | TGGAGGCACACAAAATCAACGACCG |
| GZMA | ENSSSCG00000016902 | GGAGCTCACTCGATAACCAAGAAA | GCTTTAGAAGTTTAAGGTCACCCTCAT | TCCTTATCCATGCTTTGACCAGGACACAC |
| IFNA1 | GQ415055 (GenBank) | TCAGCTGCAATGCCATCTG | AGGGAGAGATTCTCCTCATTTGTG | TGACCTGCCTCAGACCCACAGCC |
| IL1A | ENSSSCG00000008090 | CTGAAGAAGAGACGGTTGAGTTTAAA | AAGTTGTATTTCATGTTGCTCTGGAA | CAGAAGAAGAAATCATCAAGCCCAGATCAGC |
| IL8 | ENSSSCG00000008953 | CCGTGTCAACATGACTTCCAA | GCCTCACAGAGAGCTGCAGAA | CTGTTGCCTTCTTGGCAGTTTTCCTGC |
| IRF1 | ENSSSCG00000014277 | AATCCAGCCCTGATACCTTCTCT | GGCCTGTTCAATGTCCAAGTC | TGCCTGATGACCACAGCAGCTACACA |
| ITGB7 | ENSSSCG00000000257 | TCGCAGCCCAGAGTTTGACT | GGCACTGGTGACAGCAAAGA | TCAGGTAGCCCAGGCCCTCTCTGC |
| JARID2 | ENSSSCT00000001155 | CCTTTCTCTGCCTTCGAGGTT | CGTCCTGAGAGCTTCCGAAAT | TCCTGCGCTGCCCAACAGCA |
| MERTK | ENSG00000153208 | GGAAAGATGGGAAGGAATTGC | TCATCTTACAGATATATGACCCATTGTCT | TTCAGCATAACCAGTGTGCAGCGTTCA |
| PYCARD | ENSG00000103490 | CAAACCAGCACTGCACTTCGT | CAGCCCGTCCACGTCTGT | CGGGCAGCCCTCATCTCAAGGG |
| RASGRP1 | ENSSSCG00000004791 | GGAGAATAAAGAATCCCTCATAAAATCA | TTATTTCCTGTTCCAGCTCTTGGT | CTCCGTCACCTCAGACTCCCCACC |
| RPL32 | ENSSSCG00000027637 | GGAAAGATGGGAAGGAATTGC | TCATCTTACAGATATATGACCCATTGTCT | TTCAGCATAACCAGTGTGCAGCGTTCA |
| SLA-DQA1 | ENSSSCG00000001456 | GGTTCCTGAGGTGACTGTGTTT | GACAGAGTGCCCGTTCTTCAA | CTGGGTCAGCCCAACACCCTCAT |
| SLA-DRA | ENSSSCG00000001453 | CCCGCCAGTGGTCAATGT | AGTGGAACTTGCGGAAAAGG | AGGAGTGTCAGAGACAGTCTTCCTGCCC |