Table 1. Binding of 30 mutant and wild-type toxins for BtR175 obtained by fitting the curves indicated in Figures 2 and 5 (wild-type) to a 1:1 Langmuir binding models.

Clone names	Ka (×105 M−1·s−1)	kd (× 10−3·s−1)	KD (×10−8 M)
Wild-type	0.96	2.66	2.77
G275EGG278	1.15	1.66	1.45
D278DIY281	1.12	2.40	2.14
F280RWE283	0.85	1.92	2.24
A310QMA313	1.12	1.80	1.6
K310ASR313	2.51	6.75	2.69
L365EAQ368	2.57	4.28	1.66
H365AGG368	2.38	4.39	1.84
F365EPK368	0.84	3.1	3.68
F367SQA370	1.26	4.14	3.29
A367PGP370	0.94	3.82	4.06
S367TRS370	1.27	2.26	1.77
T367LGP370	0.88	4.00	4.54
L367PRP370	1.34	1.78	1.33
L367PAP370	0.85	2.55	2.98
P367RRP370	1.26	1.53	1.21
S367QAA367	0.71	2.6	3.64
H367NAG370	0.71	1.82	2.55
A367PAP370	0.81	2.7	3.31
S367PSA370	0.79	2.93	3.68
S371APN374	1.25	1.67	1.33
E371PRH374	2.90	3.28	1.13
R375GPD378	1.85	1.22	0.65
V375GCA378	1.06	3.01	2.84
G435LVG438	4.14	5.59	1.35
S435VVG438	1.90	3.79	1.99
E439PPA442	1.21	1.40	1.16
T439LRT442	1.51	1.58	1.05
D439TGN442	1.20	1.62	1.35
I443VPV446	1.58	3.16	1.99
V443ELL446	1.33	2.72	2.04