Table 2. BtR175 binding by mutant toxins containing two mutated loops in one molecule.

Values were obtained by fitting the curves indicated in Figure 5 to a 1:1 Langmuir binding model.

Clone names	ka * (×105 M−1·s−1)	kd* (×10−3·s−1)	KD (×10−8 M)
Wild-type	0.960	2.66	2.77
Q439PRG442 †	7.96†	1.62†	0.211†
H439MPR442 †	15.9†	1.05†	0.066†
R443LGR446 †	7.12†	1.32†	0.185†
R375GPD378/PQ439RG442	5.13	1.75	0.340
R375GPD378/H439MPR442	21.6	1.22	0.056
R375GPD378/R443LGR446	6.46	1.75	0.270

*To calculate parameters, the 1:1 Langmuir binding fit model was applied to the curves indicated in Figure 5.

†Y. Fujii, S. Tanaka, M. Otsuki, Y. Hoshino, H. Endo and R. Sato, unpublished work.