Table 2.
A. otitidis isolates cultured from eardischarge swabs
| Sample |
Days of culture until
A. otitidis
was isolated |
Penicillin MIC | Erythromycin MIC | Azithromycin MIC | |
|---|---|---|---|---|---|
| HBA agar | BHI agar with 6.5%NaCl | ||||
| Child A (Left ear) |
5 |
7 |
0.19 |
4 |
1.5 |
| Child A (Right ear) |
2 |
5 |
0.125 |
3 |
2 |
| Child B |
14 |
5 |
0.006 |
>256 |
128 |
| Child C |
Not detected* |
9 |
0.19 |
>256 |
>256 |
| Child D | 7 | Not detected | 0.032 | 96 | 64 |
Culture and antibiotic susceptibility data for five isolates from four children (designated child A-D). All cultures were positive for other bacteria after two days’ incubation. MIC values are in μg/mL. In the absence of A. otitidis-specific CLSI breakpoints, the MIC values used to define pneumococcal susceptibility were applied [28]. For oral penicillin V: sensitive ≤0.06μg/mL, intermediate susceptibility 0.12-1μg/mL, resistant ≥2μg/mL. For erythromycin: sensitive ≤0.25μg/mL, intermediate susceptibility 0.5μg/mL, resistant ≥1μg/mL. For azithromycin: sensitive ≤0.5μg/mL, intermediate susceptibility 1μg/mL, resistant ≥2μg/mL. HBA = Horse blood agar. *This plate was overgrown by a Proteus sp.