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Steady-state kinetic parameters for the AlgL reaction.
| substratea | kcat/Km (s−1M−1) | kcat (s−1) | Km (μM) |
|---|---|---|---|
| polyM | 2.5±0.6 × 106 | 32±3 | 13±3 |
| polyM-OAc | 0.30±0.06 × 106 | 1.5±0.1 | 5±1 |
| alginate | 3.1±0.9 × 106 | 32±4 | 11±3 |
| alginate-OAc | 0.5±0.1 × 106 | 1.2±0.1 | 2.6±0.7 |
PolyM and polyM-OAc used in the experiment were dp 133; alginate and alginate-OAc were dp 263.
