Figure 5.
Typical current traces from a ~7 nm nanocrystalline Al2O3 pore after the addition of 5 kbp dsDNA at a concentration of 6 nM to the cis side (anode) of the setup at applied voltages of (a) 100 mV (b) 300 mV (c) 500 mV. Distinct downward current blockades were observed (Insets) Negative controls at each voltage: Pore current is steady prior to the introduction of dsDNA, no blockades were seen.