Fig. 5.

Evolutionary profile of active site residues in HKs. Derived from the sequence alignment of 150 HK homologs and rendered onto HK853 from Thermotoga maritima. Red colored residues/areas indicate high degrees of evolutionary conservation while blue regions indicate variations in conservation. Inset: Co-crystallized structure of ADP in the CA domain of HK853¹³ illustrating residues that are involved in binding of the nucleotide. The measured distance between the exocylic N of ADP and D411 is 2.83 Å.