Eticlopride pretreatment attenuates the METH-induced DA uptake decrease in VMAT-2M vesicles as assessed 24 h after METH treatment. Rats received four injections of METH (7.5 mg/kg/injection, s.c., 2-h intervals) or saline vehicle (1 ml/kg/injection, s.c., 2-h intervals) and were killed 24 h later. Thirty minutes before each injection, rats were pretreated with eticlopride (ETIC; 0.5 mg/kg/injection, i.p.) or saline vehicle (1 ml/kg/injection, i.p.). Each column represents the mean ± S.E.M. of 12 independent determinations. *, indicates a statistical difference from saline/saline treated animals; +, indicates a statistical difference from saline/METH treated animals.