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(A) Activity of hxfA, hxfB, and rrsH-phoA transcriptional fusions (designated XfHA, XfHB, and XfR) during growth of X. fastidiosa on solid PD3 medium. (B) Induction of XfHA and XfHB in response to XfDSF. X. fastidiosa wild-type (WT) cells or an rpfF mutant were grown for 5 days on PD3 plates, and then the plates were overlaid with a fresh PD3 layer onto which the bioreporter strains were inoculated. AP (alkaline phosphatase) activity was measured after 24 to 48 h.
