Fig. 2.

Depletion of FDX1 results in iron accumulation within mitochondria. After fractionation, the iron contents were determined (described in Materials and methods), and are displayed in the unit of μg/dl [Fe²⁺]. Data represent the mean ± SE (n=3). Analysis of variance (ANOVA) was used for the statistical analysis, with P-value <0.05 considered to be significant. The results shown are representative of data from three independent experiments that displayed similar results. Statistical analyses of the data from these three experiments showed that there was no significant difference between wild-type and negative control, since the mitochondrial iron contents were 4.9 ± 0.02 and 4.8 ± 0.03 under the normal medium culture condition, whereas, mitochondrial iron contents were 5.52 ± 0.01 and 5.48 ± 0.02 when cells were grown in FAC-supplemented medium. However, the mitochondrial iron contents were 11.04 ± 0.02 for oligo1-treated cells, 9.08 ± 0.03 for oligo2-treated cells under the conditions of normal medium culture, and with FAC supplementation, the mitochondrial iron contents were 89.17 ± 0.49 for oligo1-treated cells and 78.15±0.62 for oligo2-treated cells. These values are significantly different (P<0.05) from those of RNAi oligo control-treated cells, as calculated by ANOVA followed by two-tail Dunett's test.