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. Author manuscript; available in PMC: 2013 Jan 16.
Published in final edited form as: Biochim Biophys Acta. 2011 Nov 10;1823(2):484–492. doi: 10.1016/j.bbamcr.2011.11.002

Fig. 5.

Fig. 5

FDX1 is essential for heme biosynthesis in the human erythoblast cell line, K562. (A) Both FDX1 protein and mRNA levels were decreased upon FDX1 depletion in K562 cells. (B) Compared with wild-type and negative control K562 cells, FDX1 knock-down led to increased IRP2, TfR1 and FPN protein expression, whereas FTN protein expression levels decreased. (C) At mRNA levels, FDX1 knock-down led to increased expression of TfR1 mRNA, but there was no change of FTN mRNA. MFRN mRNA levels increased. (D) Decreased heme content was detected in FDX1-depleted K562 cells compared with wild-type and negative control K562 cells. (E) Upon FDX1 depletion, FECH and HMOX1 protein levels increased, whereas ALAS2 protein levels decreased. (F) Upon FDX1 depletion, FECH mRNA level increased.