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. Author manuscript; available in PMC: 2013 Jan 16.
Published in final edited form as: Biochim Biophys Acta. 2011 Nov 10;1823(2):484–492. doi: 10.1016/j.bbamcr.2011.11.002

Fig. 6.

Fig. 6

siRNA treatments of the sole human ferredoxin reductase, FDXR, cause decreased iron–sulfur protein activities and activation of IRPs. Using oligonucleotides, we decreased FDXR protein (6A) and mRNA levels (6B, left). After three successive transfections, activities of m-aco and c-aco were reduced. This experiment is representative of three experimental repeats. IRE binding activities of IRP1 and IRP2 increased on gel-shift assay (6D) and xanthine oxidase activity decreased significantly (6E), whereas lactate dehydrogenase activity did not change (6F).