Fig. 8.
FDXR knockdown resulted in cytosolic iron depletion and increased expression of the mitochondrial iron importer, MFRN1. On western blots, we observed increased IRP2 and TfR1, decreased ferritin (FTN), and increased mitoferrin (MFRN1) (8A). On western blot, we also noted decreased NTHL, an iron–sulfur cluster containing endonuclease and decreased cytochrome C, a mitochondrial heme protein (8B). Complex I activity was significantly decreased in FDXR knockdowns (8C).
