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. 2012 Nov 30;4(12):1887–1897. doi: 10.3390/nu4121887

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Effects of LP on PGE2 production (A) and calcium release (B) in LPS-stimulated RAW 264.7 macrophages. PGE2 concentrations in the culture medium were measured after 24 h incubation by a PGE2 parameter assay. And, intracellular calcium release was measured after 30 min incubation by Fluo-4 assay. Normal group (Nor) was treated with medium only. Control group (Con) was treated with LPS (1 μg/mL) alone. Indo denotes indomethacin (0.5 μM) with LPS. Values are the mean ± SEM of three independent experiments. * p < 0.05 vs. Con.