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. 2012 Nov 28;13(12):15967–15982. doi: 10.3390/ijms131215967

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Single filament binding assay. Trx-PT, trx-PT(1–129) or trx-PT(125–165) was conjugated with Alexa-488, which emitted green fluorescence after excitation. Actin filaments were labeled with rhodamine-phalloidin, which emitted red fluorescence after excitation. Alexa-488-labeled trx-PT(1–129) does not bind to actin filaments. Alexa-488-labeled trx-PT(125–165) binds to the sides of actin filaments. Alexa-488-labeled trx-PT binds to the end of actin filaments. Under assay conditions, 96% of Alexa-488-labeled trx-PT were localized at the filament end (n = 52); 98% of Alexa-488-labeled trx-PT(125–165) were bound to the sides of actin filaments (n = 92). The bar represents 3 μm.