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. 2012 Nov 28;13(12):15967–15982. doi: 10.3390/ijms131215967

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Trx-PT(125–165) and trx-PT(1–129) cannot impede the elongation rates of pointed ends. (a) Trx-PT(125–165) and trx-PT(1–129) cannot retard the pointed end elongation of gelsolin-actin seeds. However, under the same conditions, trx-PT and trx-PT(35–165) impede the elongation rates of pointed ends. The bar represents 3 μM; (b) The elongation rates (mean ± SD) of pointed ends in the presence of buffer (1.66 ± 0.16 μm/min, n = 50), trx-PT in buffer (0.34 ± 0.07 μm/min, n = 60), trx-PT(35–165) in buffer (0.36 ± 0.05 μm/min, n = 69), trx-PT(125–165) in buffer (1.64 ± 0.17 μm/min, n = 65) or trx-PT(1–129) in buffer (1.67 ± 0.13 μm/min, n = 50). The lengths of the growing actin filaments (green fluorescence) were obtained from data presented in (a).

Trx-PT(125–165) and trx-PT(1–129) cannot impede the elongation rates of pointed ends. (a) Trx-PT(125–165) and trx-PT(1–129) cannot retard the pointed end elongation of gelsolin-actin seeds. However, under the same conditions, trx-PT and trx-PT(35–165) impede the elongation rates of pointed ends. The bar represents 3 μM; (b) The elongation rates (mean ± SD) of pointed ends in the presence of buffer (1.66 ± 0.16 μm/min, n = 50), trx-PT in buffer (0.34 ± 0.07 μm/min, n = 60), trx-PT(35–165) in buffer (0.36 ± 0.05 μm/min, n = 69), trx-PT(125–165) in buffer (1.64 ± 0.17 μm/min, n = 65) or trx-PT(1–129) in buffer (1.67 ± 0.13 μm/min, n = 50). The lengths of the growing actin filaments (green fluorescence) were obtained from data presented in (a).