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. 2012 Dec 5;13(12):16624–16635. doi: 10.3390/ijms131216624

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Apoptosis induced by plasma concentrations of drugs in NSCLC cell lines. (A) Percentage of apoptosis induced by Pac, Car and Pac-Car combination therapy. Values represent means ± SD of quadruplicate cultures; (B) To confirm apoptotic induction, cells were analyzed using annexin V-FITC staining and confocal microscopy. The figure shows representative images of stronger staining in A-549 cells (a: annexin V-FITC staining; b: cell nuclei counterstained with PI; c: overlay) and weak staining in NODO cells (d: annexin V-FITC staining; e: cell nuclei counterstained with PI; f: overlay). The study was carried out at 48 h. Scale bar: 25 μm.