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. 2012 Dec 14;13(12):17138–17159. doi: 10.3390/ijms131217138

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Scheme of SELEX procedure (Systematic Evolution of Ligands by EXponential enrichment) for ergot alkaloids. (1) Adsorption. A random 80-base ssDNA library is incubated with the ergot alkaloid coated magnetic beads. By applying a magnetic stand, the fraction of ssDNA which is not bound to the ergot alkaloid coated magnetic beads is discarded. (2) Recovery. After washing steps, the fraction of ssDNA bound to the ergot alkaloid coated magnetic beads is eluted from the ergot alkaloid coated magnetic beads. (3) Amplification. The selected ssDNA aptamers are amplified by polymerase chain reaction (PCR) in order to be used as input for the following selection cycle.