Table 3.

Optimized parameter values

				Optimized Parameter Value withWidth Of 95% Confidence Interval Indicated Between Parentheses (%)
Parameter	Unit	Description	Initial Condition	Model conf. i	Model conf. ii	Model conf. iii
kATP Hydr (0 Hz)	s−1	ATPase rate constant 0 Hz	0.001a	0.0020 (±3.8%)	0.0020 (±5.1%)	0.0017 (±2.0%)
kATP_Hydr (5 Hz)	s−1	ATPase rate constant 5 Hz	0.01a	0.084 (±6.4%)	0.091 (±6.8%)	0.068 (±3.1%)
kATP_Hydr (10 Hz)	s−1	ATPase rate constant 10 Hz	0.01a	0.072 (±5.9%)	0.077 (±6.4%)	0.056 (±3.1%)
kATP_Hydr (40 Hz)	s−1	ATPase rate constant 40 Hz	0.01a	0.060 (±5.3%)	0.065 (±6.0%)	0.049 (±2.8%)
kATP_Hydr (80 Hz)	s−1	ATPase rate constant 80 Hz	0.01a	0.047 (±9.8%)	0.050 (±10.2%)	0.041 (±3.9%)
BuffCapFixed	M	Fixed buffer cellular proton buffer capacity	0.0142b	0.068 (±8.6%)	0.073 (±10.8%)	0.050 (±5.5%)
VmaxPFK	mM/s	Vmax PFK	0.93b	8.76 (±26.0%)	10.43 (±43.8%)	10.25 (±11.9%)
KAMP	mM	PFK deinhibition constant AMP	0.002c	0.0017 (±29.1%)	0.014 (±5.2%)	0.046 (±23.3%)
KADP	mM	PFK deinhibition constant ADP	0.030c	0.31 (±49.3%)	0.16 (26.6±%)	0.24 (±7.2%)
kdeinhib_Act	unitless	PFK deinhibition constant activated PFK	1c	—	8.72 * 106 (±2.45 * 105%)	—
KAMPact	mM	PFK deinhibition constant AMP activated PFK	0.002c	—	—	2.23 * 10−5 (±6.0%)
KADPact	mM	PFK deinhibition constant ADP activated PFK	0.030c	—	—	0.020 (±28.3%)
kon	μM−2/s	PFK, calcium-calmodulin binding constant	1 * 104–1 * 109	—	1.26 * 106 (±2.00 * 103%)	6.08 * 105 (±19.3%)
koff	s−1	PFK, calcium-calmodulin binding constant	1 * 101–1 * 104	—	4.75 * 103 (±23.3%)	106 (±13.3%)
kATP_Hydr (1 Hz)	s−1	ATPase rate constant 5 Hz	0.005a,d	0.036 (5.0%)	0.038 (15.6%)	0.032 (5.0%)
kATP_Hydr (40 Hz, 20 pulses)	s−1	ATPase rate constant 40 Hz 20 pulses	0.02a,d	0.050 (20.0%)	0.042 (35.3%)	0.072 (11.6%)

PFK, phosphofructokinase; conf., configuration.

^aEstimated from initial PCr breakdown rate;

^bvalues used as initial condition in Ref. 55;

^cvalues obtained from Ref. 10;

^destimated for validation experiments (Fig. 5).