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The affinity of candidate epitopes for HLA-A*0201 molecules. T2 cells were incubated in RPMI 1640 medium lacking peptides (red) or containing low peptide (1 μg/ml) (green) or intermediate peptide (10 μg/ml) (purple) or high peptide (100 μg/ml) (blue) for 15 h and stained with FITC-conjugated anti-HLA-A*0201 antibody. Then, mean fluorescence intensity (MFI) was measured using flow cytometry.
