Table 1.
Titer assessment for HHV-6A GS strain batches expressed as TCID50 determined by Q-PCR, ocular inspection or immunofluorescence assay (IFA), and as infectious units (Inf U) determined by IFA
| Batch | Q-PCR | Ocular inspection | Inf U/ml | IFA | HHV-6A |
|---|---|---|---|---|---|
|
(passage) |
TCID50/ml |
TCID50/ml |
|
TCID50/ml |
DNA cop/ml |
| P17 |
1215 ±566 (x3)1 |
771 ±466 (x3) |
nd2 |
972 ±719 (x2) |
7.6e8 ±1.4e8 (x2) |
| P19 |
806 ±679 (x5) |
465 ±408 (x5) |
2.5e4 ±1.6e4 (x3) |
649 N/A3 (x1) |
16.0e8 ±1.3e8 (x2) |
| P21 |
6 ±8 (x2) |
14 ±6 (x2) |
nd |
nd |
7.7e8 ±0.15e8 (x2) |
| P27 |
7 ±6 (x3) |
30 ±15 (x3) |
3.9e4 N/A (x1) |
13 ±3 (3x) |
nd |
| P17 UV-inact. |
0 N/A (x1) |
0 N/A (x1) |
nd |
nd |
nd |
| P17 heat-inact. | 20 N/A (x1) | 4 N/A (x1) | nd | nd | nd |
1 Number of replications of titer assessments for each passage.
2 nd: Not done.
3 N/A: Not applicable.
Ocular inspection was performed by two independent assessors. Data presented are means ± standard deviations.