Table 3.
SNPs found in gyrA and gyrB genes for M. avium subsp. paratuberculosis strain K-10 and M. avium subsp. paratuberculosis types I and III
| Strains | Type | IS900 RFLP profiles |
gyrA |
gyrB |
||
|---|---|---|---|---|---|---|
| position 1822 | 1986 | 1353 | 1626 | |||
| K10* |
II |
R01 |
CCCGAGGAGCGGATCGCT- |
ACTCGTGGGCGCGGTGTTGT |
CCGGTCGACCGATCCGCGC- |
CCAGCACATCTCGACGCTGT |
| 6756 |
I |
S1 |
.....A....- |
.......... |
......C...- |
.......... |
| 6759 |
I |
S1 |
.....A....- |
.......... |
......C...- |
.......... |
| P133/79 |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| 21P |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| 235 G |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| M189 |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| M15/04 |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| M254/04 |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| M71/03 |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| M72/03 |
I |
S2 |
.....A....- |
.......... |
......C...- |
.......... |
| 22 G |
III |
A |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| OVICAP16 |
III |
A |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| OVICAP49 |
III |
A |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| 21I |
III |
B |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| PCR311 |
III |
B |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| 19I |
III |
C |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| 85/14 |
III |
C |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| OVICAP34 |
III |
D |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| 18I |
III |
E |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| FO21 |
III |
F |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| LN20 |
III |
I1 |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| 269OV |
III |
I10 |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| M284/08 |
III |
I10 |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| P465 |
III |
I2 |
.....A....- |
.....T..... |
......C...- |
.....T..... |
| Consensus |
I |
|
CCCGAGGAGAGGATCGCT- |
ACTCGTGGGCGCGGTGTTGT |
CCGGTCGACCGACCCGCGC- |
CCAGCACATCTCGACGCTGT |
| Consensus |
III |
|
CCCGAGGAGAGGATCGCT- |
ACTCGTGGGTGCGGTGTTGT |
CCGGTCGACCGACCCGCGC- |
CCAGCACATTTCGACGCTGT |
| K10 | II | CCCGAGGAGCGGATCGCT- | ACTCGTGGGCGCGGTGTTGT | CCGGTCGACCGATCCGCGC- | CCAGCACATCTCGACGCTGT | |
*K10 sequenced strain type II used as sequence reference.