Table 4.
Primer details
| Name | Gene | Nucleotide position | Sequence (5’-3’) | Amplicon length (bp) | Annealing temp. (°C) |
|---|---|---|---|---|---|
| LongF1 |
trnF_atp6/atp6 |
28-49 |
ACTGAAGATGCTGAGATGAGCC |
7961 |
55 |
| LongR2 |
8012-8033 |
ATGGTCAGTTTCAAGGGTTAGG |
|||
| LongF2 |
atp6/atp6_trnF |
8012-8033 |
CCTAACCCTTGAAACTGACCAT |
9649 |
55 |
| LongR1 |
28-49 |
GGCTCATCTCAGCATCTTCAGT |
|||
| 16SA-Lmod |
rrnL |
1943-1962 |
CGCCTGTTTACCAAAAACAT |
580 |
53 |
| 16SA-H |
|
2542-2562 |
CCGGTCTGAACTCAGATCACG |
|
|
| COX1F |
cox1 |
6613-6631 |
GAAACATGAGCAAAAATCC |
190 |
53 |
| COX1R |
|
6821-6802 |
AATGCTTCTCAGATAATGAA |
|
|
| ND6F |
nad6 |
13996-14015 |
AACATCCCACCTAAATAAAT |
106 |
53 |
| ND6R |
14137-14122 |
TAGCTGTTGCTTCAAATCC |
|||
| AMP1F |
trnF_rrnS |
5-23 |
ACGTAGCTTAAGTAAAGCACAGC |
294 |
58 |
| AMP1R |
322-347 |
ATCAACTTGAGTTTCTCGTATAACC |
|||
| AMP2F |
cox2_trnK_atp6/cox3 | 7776-7800 |
TCTTCATCAATACTAGAAGCCTCA |
912 | 61 |
| AMP2R | 8712-8731 | TGTGCTTGGTGTGCCATTA |
The names, gene and nucleotide positions, sequences, expected amplicon lengths and annealing temperatures of PCR primers used to generate the long-amplicons and to verify the specific identity of the long-amplicon and sequences of the primer regions.