Skip to main content
. 2013 Jan 16;8(1):e54054. doi: 10.1371/journal.pone.0054054

Figure 5. Maintenance of PGC1α levels protects against TWEAK-induced contractile dysfunction in isolated cardiomyocytes.

Figure 5

Isolated cardiomyocytes were infected with adenovirus (MOI of 10) expressing GFP or PGC1α for 24 hours prior to treatment with IgG or rTWEAK for 48 hours. (A) Western blot analysis of PGC1α expression, normalized with GAPDH. N = 3 independent biological replicates group. (B) Isolated cardiomyocyte function (%CS) was determined using edge detection method. (C) Representative tracings of single cell shortening at indicated conditions. (D) Time to 90% relaxation in isolated cardiomyocytes. Cellular function was assessed in three independent biological replicates and data from 8–12 cells was averaged as N = 1 for a given experiment. * p<0.05 vs. IgG and # p<0.05 vs. rTWEAK in Ad-GFP group.