Figure 1. In vitro growth of RR mutants.

Growth of all 13 RR mutants with Erm-resistance cassettes, and control mutant Δsp_1678 with Erm- and Km-resistance cassettes. Bacterial cultures were individually grown at 37°C in microaerophilic conditions over the course of 10 hrs. Growth was measured by OD readings at 4 hrs and every hour thereafter on a FLUOstar plate reader at 600 nm.