Table 3. Endocarditis virulence of mutants determined by qPCR-CI.

Mutant	CI (Mean1± SD)	P value2	Protein name	TCS family	TCS	Virulence3
Δsp_0083	2.00±1.37	0.15	DNA-binding response regulator	Pho	TCS08	y
Δsp_0156	0.47±0.26	0.04	DNA-binding response regulator	Lyt	TCS07	y
Δsp_0376	8.57E-05±1.7E-05	7.70E-06	DNA-binding response regulator	Pho	TCS14	y
Δsp_0387	0.66±0.75	0.72	DNA-binding response regulator	Nar	TCS03	n
Δsp_0526	1.01±0.29	0.79	response regulator BlpR	Agr	TCS13	y
Δsp_0603	1.04±0.56	0.68	DNA-binding response regulator VncR	Pho	TCS10	n
Δsp_0661	0.98±0.36	0.83	DNA-binding response regulator	Lyt	TCS09	y
Δsp_0798	0.0026±0.0014	7.40E-10	DNA-binding response regulator CiaR	Pho	TCS05	y
Δsp_1633	0.44±0.16	0.0061	DNA-binding response regulator	Pho	TCS01	y
Δsp_2000	2.36±1.37	0.11	DNA-binding response regulator	Nar	TCS11	n
Δsp_2082	0.33±0.21	0.01	response regulator	Pho	TCS04	y
Δsp_2193	3.75±1.72	0.04	DNA-binding response regulator	Pho	TCS06	y
Δsp_2235	0.95±0.35	0.95	response regulator ComE	Agr	TCS12	y
SP_1227	Essential gene		DNA-binding response regulator	Pho	TCS02	y

¹Mean, geometric mean.

²P values determined by one-sample t test with false discovery rate control.

³Virulence cited from Paterson et al. [16].