Figure 3. GSK3 inhibitors inhibit osteoblast differentiation in ADSCs.

(A) ADSCs were grown to confluence and then cultured in osteogenic differentiation medium with and without 5 μΜ SB216763 (SB21) or 20 mM lithium chloride (LiCl) for 12 days. Subsequently, cells were subjected to ARS (upper) and VK (lower, VK) staining. (B) Total RNA was isolated from cells as in (A) and analyzed for ALP, osterix, and osteopontin expression by real time PCR. DMSO and NaCl were used as the vehicle control. Data represent mean ± S.D. and are representative of at least 3 experiments. *p≤0.01, significantly different from without GSK3β inhibitors group.