Table 1. Primer sequences for Reverse transcription PCR and real time PCR analysis.
| Primer sequence (5′ - 3′) | ||
| Gene | Forward | Reverse |
| ALP | GCCCTCTCCAAGACATATA | CCATGATCACGTCGATATCC |
| Osterix | CTGGGGAAAGGAGGCACAAAGAAG | GGGTTAAGGGGAGCAAAGTCAGAT |
| Osteopontin | CAGTGATTTGCTTTTGCCTGTTTG | GGTCTCATCAGACTCATCCGAATG |
| Adipsin | ATGGTATGATGTGCAGAGTGTAG | CACACATCATGTTAATGGTGAC |
| PPARγ2 | GGGTGAAACTCTGGGAGATTCTC | TCAGCAACCATTGGGTCAG |
| GSK3β | CTTGGACAAAGGTCTTCCGGCC | GTTGGCAGGCGGTGAAGCAG |
| β-catenin | TGCTGAAGGTGCTGTCTGTC | CTGCTTAGTCGCTGCATCTG |
| GAPDH | CCCTTCATTGACCTCAACTAC | CCAAAGTTGTCATGGATGACC |
| * ALP | GCTGATCATTCCCACGTTTT | CTGGGCCTGGTAGTTGTTGT |
| * Osterix | TGAGGAAGAAGCCCATTCAC | ACTTCTTCTCCCGGGTGTG |
| * Osteopontin | CGATGTCCCCAACGGCCGAG | TGCTCAGAAGCTGGGCAACAGG |
| * β-Actin | GCTTCTTTGCAGCTCCTTCGT | ATCGTCATCCATGGCGAACT |
*
Primer sequences for real time PCR analysis.