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. 2013 Jan 16;8(1):e54563. doi: 10.1371/journal.pone.0054563

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© 2013 Ding et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Total mRNA extracted from heart, lung, liver, kidney, stomach, small intestine, brain and spinal cord of adult rats was assayed by quantitative real-time PCR (n = 5). Relative quantification was assessed by normalizing the amount of Nischarin to the housekeeping gene GAPDH. (B, C) Protein samples from different tissues were analyzed by Western blot and the quantitative analysis was performed by normalizing the intensities of the hybridization signals to GAPDH (n = 5). Nischarin is highly expressed in liver, brain and spinal cord at both the mRNA and protein levels. Data are presented as mean ± SD.