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. 2013 Jan 16;8(1):e54524. doi: 10.1371/journal.pone.0054524

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© 2013 Raynaud et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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After culture on matrigel in mTESR1 media undifferentiated hESCs were shifted to MP specific media for 6 days. After 6 days the confluent culture of differentiated cells were split to 1/3 with dispase and replated on matrigel. After an extra 6 days of culture, differentiated cells were plated on coating-free feeder-free culture dishes. Cells were analyzed for MP markers expression at each step of the process. B. Phase contrast imaging of cell morphology modification during the derivation process of hESC-MPs. C. Chart representing flow cytometry analysis of mesenchymal progenitor markers CD29, CD90, CD73 and CD105 at the different time-points of our differentiation process.