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. 2013 Jan 16;8(1):e53912. doi: 10.1371/journal.pone.0053912

Figure 3. Ex vivo expanded CD34+CD38 −CD90+ cells demonstrate high preservation of the HSC transcriptional program.

Figure 3

(A) Hierarchical clustering using spearman rank correlation and (B) principal component analysis comparing freshly isolated CD34+CD38−CD90+ cells (Day 0) to CD34+CD38+CD90− differentiated progenitors and to ex-vivo expanded CD34+CD38−CD90+ cells demonstrate high conservation of the HSC transcriptome during culture on OP9M2 stroma. (C) Number of genes with more than 2-fold change in gene expression compared to Day 0 CD34+CD38−CD90+ cells is shown. (D) Graph showing the relative expression of transcription factors known to regulate HSC development or maintenance. (E) Graph showing the relative expression of transcription factors known to initiate hematopoietic lineage commitment. Values represent a fold difference to freshly isolated CD34+CD38−CD90+ cells. Raw data is available for download from Gene Expression Omnibus (http://ncbi.nlm.nih.gov/geo) (GSE34974).