Table 1. Real-time PCR primers used in this experiment.
Target gene | Primer Sequence (5'-3') | Size(bp) | |
Forward | Reverse | ||
β-actin | CGTTGACATCCGTAAAGACCTC | TAGGAGCCAGGGCAGTAATCT | 110 |
β-tubulin | GGAGCTGTTCAAGCGC | AGGTCATTCATGTTGCTCTC | 129 |
vimentin | GGACCTGCTCAATGTAAAGATG | GGTGTCAACCAGAGGAAGTG | 157 |
ezrin | GCTCCTGACTTCGTGTTCTAC | AGTTTCTCTCCTCTTCTTCTCG | 207 |
radixin | ATGTGAGAGCCGTTCTTGTG | CAACTGTCTGAGTGCTTTGG | 117 |
moesin | GGACCAGAAGAAGACTCAGG | CATTCCTCAGCCTCACTCTC | 111 |
stathmin | GAGGAGAACAACAACTTCAGC | TCGGAACAACTTAGTCAGCC | 199 |
COX I | TGAGCAGGAATAGTAGGGACA | GAGTAGAAATGATGGAGGAAGC | 261 |
COX II | CTTATTCTTATCGCCCTCCC | GCTTCAGTATCATTGGTGACC | 105 |
COX III | CAAGCCCTACTAATCACCATTC | GAAATCCCGTTGCTATGAAG | 130 |
ND1 | GCCACATCAAGTCTTTCAGTC | ATTTGTAGGGAGAAGGAGCC | 173 |
ND3 | CGACCTAGAAATCGCCTTAC | CATTCGTAGCTTAGGCCAAG | 123 |
ND6 | GTTAGTGGATGTATTGGGTGC | CCTCAGTAGCCATAGCAGTTG | 130 |
ATPase6 | GCAACCGACTACACTCATTTC | GGGATGGCTATGCTTAGGTC | 205 |
ATPase8 | TCATCTCCTCAATAGCCACAC | TGATTCTCAAGGGTTATTCGT | 122 |
GAPDH | CGCTAACATCAAATGGGGTG | TTGCTGACAATCTTGAGGGAG | 201 |