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. Author manuscript; available in PMC: 2013 Jan 16.
Published in final edited form as: Angiogenesis. 2008 Sep 17;11(4):347–360. doi: 10.1007/s10456-008-9116-2

Fig. 1.

Fig. 1

TNP-470, a β-catenin-independent Wnt signaling inhibitor, selectively disrupts endothelial cell polarity. a Typical caveolin-1 (green) polar localization to the trailing edge (white arrowheads) of migrating MPE endothelial cells (DMSO control). b Upon the addition of 10 nM TNP-470, caveolin-1 localizes throughout the cytosol (and is no longer polarized to the trailing edge of the cell). c Proportion of MPE cells displaying a polar localization of caveolin-1 (% Polar) in the presence or absence of a Wnt5a-gradient and/or 10 nM TNP-470 (N = 3–5 Dunn chemotaxis chambers, each N = 12 fields of view (FOV)). d Depolarizing activity of TNP-470 is rescued upon activation of β-catenin-independent signaling by ΔDIX-Dvl2 (N = 3–5 Dunn chemotaxis chambers, each N = 12 FOV) (P >0.05). e Polarization of caveolin-1 in NHDF cells in Dunn chemotaxis chambers. The addition of TNP-470 did not disrupt caveolin-1 localization in NHDF cells (P >0.05). Dashed lines encircle cells to facilitate their visualization