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. 2013 Jan;48(1):27–34. doi: 10.1165/rcmb.2012-0187OC

Figure 1.

Figure 1.

Ozone alters the inflammatory response to the synthetic Toll-like receptor–2 (TLR2)/TLR1 agonist Pam3CYS in murine whole-lung lavage (WLL). (A) Cell counts and (B) selected cytokines and chemokines in the WLL of C57BL/6 mice in response to intratracheal instillation of saline or Pam3CYS 24 hours after ozone (2 ppm, 3 h) or filtered air (FA) exposure. Measurements were performed at two time points, 4 hours and 24 hours after Pam3CYS instillation (28 h and 48 h after ozone exposure, respectively). Data are expressed as mean ± SEM (n = 10–15 C57BL/6 mice from three independent experiments). Group comparisons included O3/saline versus FA/saline, O3/saline versus O3/Pam3CYS, FA/Pam3CYS versus FA/saline, and O3/Pam3CYS versus FA/Pam3CYS. Statistical significance was determined using the Mann-Whitney U test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P < 0.0001).