TABLE 2.
EFFECTS OF OZONE ON RL AND EL SPECTRA IN SFTPD+/+ AND SFTPD−/− MICE
|
Sftpd+/+ |
Sftpd−/− |
||||
| PEEP | Air | Ozone | Air | Ozone | |
| a | 0 | 3.39 ± 0.11 | 3.62 ± 0.19 | 3.69 ± 0.16 | 8.71 ± 3.41 |
| 1 | 3.50 ± 0.05 | 3.53 ± 0.19 | 3.52 ± 0.32 | 8.09 ± 3.76 | |
| 3 | 3.20 ± 0.07 | 2.91 ± 0.22 | 3.32 ± 0.20 | 3.12 ± 0.71 | |
| 6 | 3.20 ± 0.13 | 2.59 ± 0.18 | 2.89 ± 0.19 | 3.16 ± 0.25 | |
| b | 0 | 0.08 ± 0.01 | 0.13 ± 0.04 | 0.24 ± 0.04* | −0.06 ± 0.09† |
| 1 | 0.10 ± 0.02 | 0.20 ± 0.04† | 0.29 ± 0.04* | 0.13 ± 0.04† | |
| 3 | 0.19 ± 0.01 | 0.43 ± 0.02† | 0.45 ± 0.01* | 0.73 ± 0.17 | |
| 6 | 0.34 ± 0.01 | 0.45 ± 0.01 | 0.66 ± 0.01* | 0.57 ± 0.06 | |
| c | 0 | 0.58 ± 0.03 | 0.70 ± 0.06 | 0.62 ± 0.05 | 0.76 ± 0.19 |
| 1 | 0.67 ± 0.03 | 0.68 ± 0.04 | 0.60 ± 0.04 | 1.05 ± 0.33 | |
| 3 | 0.61 ± 0.01 | 0.45 ± 0.05 | 0.67 ± 0.13 | 0.31 ± 0.16 | |
| 6 | 0.58 ± 0.02 | 0.30 ± 0.02† | 0.37 ± 0.03* | 0.29 ± 0.03 | |
| E0 | 0 | 61.36 ± 1.21 | 63.75 ± 1.35 | 54.90 ± 0.96* | 66.46 ± 4.92 |
| 1 | 62.08 ± 1.11 | 64.04 ± 1.22 | 56.62 ± 1.95* | 69.26 ± 2.40† | |
| 3 | 59.18 ± 1.31 | 64.04 ± 2.43 | 53.66 ± 2.15 | 73.88 ± 4.20† | |
| 6 | 52.45 ± 1.35 | 57.37 ± 3.41 | 51.19 ± 2.89 | 65.24 ± 3.25† | |
| ΔE | 0 | 34.57 ± 0.85 | 46.92 ± 2.75† | 36.55 ± 0.83 | 130.54 ± 56.58† |
| 1 | 32.99 ± 0.65 | 44.60 ± 1.59† | 31.16 ± 1.75 | 115.18 ± 60.07† | |
| 3 | 31.43 ± 0.77 | 30.61 ± 1.73 | 30.12 ± 2.69 | 57.01 ± 19.96 | |
| 6 | 29.02 ± 0.81 | 27.52 ± 1.92 | 26.18 ± 2.29 | 32.59 ± 2.46 | |
| β | 0 | 0.20 ± 0.00 | 0.15 ± 0.02† | 0.27 ± 0.05 | 0.18 ± 0.03† |
| 1 | 0.24 ± 0.01 | 0.16 ± 0.02† | 0.30 ± 0.03 | 0.14 ± 0.03† | |
| 3 | 0.27 ± 0.01 | 0.22 ± 0.02 | 0.30 ± 0.03 | 0.21 ± 0.04 | |
| 6 | 0.41 ± 0.01 | 0.44 ± 0.03 | 0.56 ± 0.05* | 0.48 ± 0.03 | |
Definition of abbreviations: a, b, and c are parameters of RL; a and c reflect RL at low frequencies; b reflects RL at high frequencies. E0, ΔE, and β are parameters of EL; EL, elastance spectra curve; RL, resistance spectra curve.
E0 represents EL at 0 Hz. ΔE reflects the magnitude of EL, and β the rate of EL change with frequency. Lung function was measured 72 hours after exposure of Sftpd+/+ and Sftpd−/− mice to air or ozone. Each measurement was performed in triplicate. Values represent the means ± SEM of three experiments (n = 4–6 mice/treatment group/experiment; n = 12–18 mice/group total). Data were analyzed by two-way ANOVA.
Significantly different (P < 0.05) from Sftpd+/+ mice.
Significantly different (P < 0.05) from air-exposed control mice.