Skip to main content
. 2012 Dec;47(6):784–791. doi: 10.1165/rcmb.2012-0079OC

Figure 5.

Figure 5.

Expression of intracellular IGF-1 is increased in airway epithelial cells by exposure to aerosolized LPS. (A) IGF-1 mRNA levels in the lung tissues of mice exposed to LPS as analyzed by quantitative RT-PCR. Data are shown as mean ± SEM (n = 6 per group). *P < 0.05. (B) The IGF-1 expression in airway epithelial cells was analyzed by fluorescent immunostaining. Representative micrographs showing increased numbers of IGF-1–immunopositive cells (red) in mice exposed to 0.2 (middle panel) and 20.2 μg (right panel) compared with 0 μg (left panel) LPS. (C) Representative high-magnification images of Bcl-2– and IGF-1–immunostained lung sections from mice exposed to 20.2 μg aerosolized LPS. The panels show DAPI-stained nuclei (blue), IGF-1–positive (red), and Bcl-2–positive (green) cells. The composite image shows colocalization of IGF-1 and Bcl-2 (yellow) (scale bar, 5 μM).