Figure 5.

Requirements for CSE enhancement of EMAP II–induced apoptosis. HLMVECs were cultured in six-well tissue culture plates to approximately 80% confluence. Media were replaced with fresh EGM2-MV, and cells were sensitized with 5% CSE or AC for 4 hours. CSE and AC were removed and replaced with fresh media, and anti-CXCR3 antibody (Ab) (1 μg/ml) or anti-EMAP II Ab (3.5 μg/ml) (A), p38 inhibitor (10 μM) (B), or FeTPPS (10 μM) (C) were added. After an additional 30 minutes, EMAP II (10 μg/ml) was added, and HLMVECs were cultured for an additional 16 hours. Apoptosis was determined by FACS-based TUNEL staining, and normalized to the untreated control samples.