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Requirements for CSE-induced CXCR3 up-regulation. HLMVECs were cultured in six-well tissue culture plates to approximately 80% confluence, and cells were sensitized with 5% CSE or AC for 4 hours in the presence of p38 inhibitor (10 μM) (A) or FeTPPS (10 μM) (B). After HLMVECs were cultured for 6 hours, CXCR3 up-regulation was determined by FACS, and normalized to the isotype control samples.
