Figure 6.
Leaf position-dependent oxidative stress tolerance during HL SAA. (A) In vitro photo-oxidative stress tolerance leaf disk assay, Arabidopsis plants were either HL SAA treated at leaf position 4 (white bar, yellow circle) or remained untreated (black). Leaf disks were then taken from all leaves, including distal (gray), floated on H2O2, and assayed as in Figure 5. Photographs of the leaf disks taken after 20 h and were analyzed using ImageJ software to calculate the percentage of healthy and bleached tissue, n = 3. (B) First signs of photobleaching during the in vivo assay. (C) In vivo temporal assay of photobleaching development based on leaf position comparing HL SAA treated (top panels) and non-acclimated control plants (lower panels), n = 9.