Table 2.
Real-time RT-PCR primers and Universal ProbeLibrary probes (Roche) used for quantitative transcript analysis.
| Target sequence anotation | Gene locus | Universal Probe Library probe | Primer sequences | |
|---|---|---|---|---|
| ACS2 | AT1G01480 | 80 | F | CGACGACTTTACGAGGATGG |
| R | GCTCGGAGAAGAGGTGAGTG | |||
| AGP2 | AT2G22470 | 15 | F | GGTTGCTTCTCCTCCTCAGA |
| R | TGGAGTTAATCCAGCGGAAG | |||
| ANOCO19 | AT1G52890 | 143 | F | CAACAACGGTACTTCGTCCA |
| R | TTGTCGATCTCTTGATGAAACG | |||
| APX2 | AT3G09640 | 10 | F | TCATCCTGGTAGACTGGACAAA |
| R | CACATCTCTTAGATGATCCACACC | |||
| ATBETA AMY | AT4G15210 | 115 | F | CCCGTTTACGTTATGCTTCC |
| R | ACGTTTAAGCTGCGTTTCAAG | |||
| BCB | AT5G20230 | 3 | F | GTAGGCGACGAGCTCGAAT |
| R | TTCTGATACAACTGCCACATCA | |||
| CAM5 | AT2G27030 | 103 | F | TGTCAAAGTTATGATGGCAAAGA |
| R | GAATTGCTACTACGCTTTGCTG | |||
| CAT2 | AT4G35090 | 25 | F | TCTGGTGCTCCTGTATGGAA |
| R | TGGTAATCCTCAAGAAGGATAGGA | |||
| CYCLO | AT2G29960 | 103 | F | GGCAGTTCCTAAAACTGCAGAA |
| R | TTCCCTTGTAGTGTAGAGGTTTCC | |||
| DDF1 | AT1G12610 | 12 | F | CGGAGATGAGGCCTAAGAAG |
| R | TGCCTCTGTAAACTGGGTGA | |||
| DREB2A | AT5G05410 | 121 | F | GATTTTCAAATTTCGTCCCCTA |
| R | TGTTCTGTTTCTATCTCCACTCTGA | |||
| DTA1 | AT1G02400 | 141 | F | TCATGATGATCCTTTCAAGTTCAG |
| R | CCAAATCTCTAACCGTGCGTA | |||
| EDL3 | AT3G63060 | 28 | F | ATTGTCCGGCGAAGATCC |
| R | CAGAAGAACATGAGTTTCGCTAAC | |||
| ELIP1 | AT3G22840 | 126 | F | GCACAAAGTTTAGCGACTTGC |
| R | CGCAACGAATCCAACCAT | |||
| ELIP2 | AT4G14690 | 101 | F | CCACCACAAATGCCACAG |
| R | GCAAATCTCCAAACTTCGTACTC | |||
| ERF11 | AT1G28370 | 82 | F | CGTCAAAACCAACGAAGGTAA |
| R | ACGTCCCCATGGTCTCTTC | |||
| ERF2 | AT5G47220 | 82 | F | TTACGGAGACGGCAGTGAA |
| R | AATTTCCCCCACGGTCTCT | |||
| EX1 | AT4G33630 | 116 | F | AGAAAGAGAAGAAGATTTCTGTCAAGA |
| R | ATTTTGTCAAACCCGACAGC | |||
| GH3.3 | AT2G23170 | 148 | F | CATCACAGAGTTCCTCACAAGC |
| R | GTCGGTCCATGTCTTCATCA | |||
| GH3.5 | AT4G27260 | 148 | F | CATCTCTGAGTTCCTCACAAGC |
| R | GGAACGAACTGGCTCATCA | |||
| GPX2 | AT2G31570 | 91 | F | CCTGATGGCAAGGTCTTACAG |
| R | GCAGTTTGAATGTCCTTCTCG | |||
| GST6 | AT2G47730 | 15 | F | AAGCAAGAGGCCCACCTT |
| R | TCTTGACTCGAAAAGCGTCA | |||
| GST24 | AT1G17170 | 12 | F | AGACTTGGCCCGACAATAAC |
| R | TCCTTCTCGCCGTAACATTC | |||
| LHCB1.2 | AT1G29910 | 110 | F | CCCATTGGGTCTTGCTACC |
| R | CCGTTCTTGAGCTCCTTCAC | |||
| MYB44 | AT5G67300 | 98 | F | ACCTTCCGTTGAGCTTTTCA |
| R | AGGAAGCGGTAGCACAACAG | |||
| NCED3 | AT3G14440 | 22 | F | TCGTCGTGATAGGGTCCTG |
| R | TTCTCGTCAGACTCGTTGAAAA | |||
| NHL10 | AT2G35980 | 24 | F | GCCTTCTACGGTCCATCAGT |
| R | GTGCCCACGTCGGTAGTAG | |||
| NRT1.8 | AT4G21680 | 47 | F | TGTGCACCATGAAGAGTTGAA |
| R | TGTAACAATAGCAGCTCTATCCAAG | |||
| PIN3 | AT1G70940 | 59 | F | TCTTGGAATGGCAATGTTTAGTT |
| R | CTAACCGCCATGGCAAAC | |||
| PIN4 | AT2G01420 | 159 | F | TGCCCAAAATATTACAACAATCC |
| R | TGGGTTGAAGTGCCATGA | |||
| PIN7 | AT1G23080 | 159 | F | TGGGCTCTTGTTGCTTTCA |
| R | TCACCCAAACTGAACATTGC | |||
| PP2A | AT1G13320 | 29 | F | GACCGGAGCAACTAGGAC |
| R | AAAACTTGGTAACTTTTCCAGCA | |||
| PR2 | AT3G57260 | 111 | F | GCTTAGCCTCACCACCAATG |
| R | CCCGTAGCATACTCCGATTT | |||
| PR6-like | AT2G38870 | 155 | F | GACGAGTCGTGGTTGGTTAGT |
| R | AACTTTAGGCATCAGTAACACAGAAA | |||
| Putative calcium binding protein | AT1G21550 | 63 | F | GATGTGTTGGAACGGCTAGG |
| R | CATTCTCCCACAATCCCAAG | |||
| Putative zinc finger protein | AT2G42360 | 35 | F | AAACCAGGCTGAACTTGACTG |
| R | CCGGGGATACAACTGTTTTG | |||
| RAP2.6 | AT1G43160 | 140 | F | GGACGATGGGTCATAAGAGAGA |
| R | TGAGCTTTCACATTCTTTAGTCACA | |||
| RBCS1A | AT1G67090 | 8 | F | CGCTCCTTTCAACGGACTTA |
| R | AGTAATGTCGTTGTTAGCCTTGC | |||
| RD29a | AT5G52310 | 69 | F | ACGTCGAGACCCCGATAAC |
| R | CAATCTCCGGTACTCCTCCA | |||
| RRTF1 | AT4G34410 | 68 | F | TCGGGTATGCATTATCCTAACA |
| R | AAGCTCTTGCTCCGGTGA | |||
| TCH4 | AT5G57560 | 6 | F | GCTCAACAAAGGATGAGATGG |
| R | CCTCTTCGCATCCGTACAAT | |||
| UGT76E12 | AT3G46660 | 138 | F | TCTTTGGTTACCACTCTCTAACAAGA |
| R | CTCTTCGTCACAACATGTGAATC | |||
| UGT74E2 | AT1G05680 | 29 | F | TGTGTGGAAGGTTGGGGTA |
| R | TCTTCTCTTCTCACAAACCCATC | |||
| Unknown (F26K10.20) | AT4G28140 | 143 | F | TCGTCCTAAACCCTATTTCCAA |
| R | AAAGGGAAAGCCTCTAACGAA | |||
| Unknown (MFB16.16) | AT5G50760 | 152 | F | CAAAAGGAAAGCCGAAGAAA |
| R | GGACCAACGTAAACCGTGAA | |||
| WRKY28 | AT4G18170 | 70 | F | AGGACGGCAGCTTATACTAACG |
| R | CACTTTGTCCATATCCATAATCCA | |||
| ZAT6 | AT5G04340 | 8 | F | CTCGCGACGGAGATAGAAAC |
| R | AAGCAGAGGAGGTGAAGACG | |||
| ZAT10 | AT1G27730 | 31 | F | GGACAAAGGGTAAGCGATCTAA |
| R | AGAAGCATGAGGCAAAAAGC | |||
| ZAT12 | AT5G59820 | 103 | F | CCCACGGTGACTACGTTGA |
| R | TCAAATTGTCCACCATCCCTA | |||