Figure 10. H₂O₂ enhanced TG-mediated changes in [Ca²⁺]_i of HAECs.

Fura-2-loaded HAECs in zero Ca²⁺-ECS were left untreated (A), or treated with 100 μm H₂O₂ (B) for 5 min immediately prior to challenging with 300 nm TG. A and B, individual cell traces are segregated into two phenotypic profiles as described in the legend of Fig. 5. C and D, cumulative frequency analysis of the peak ratio (C) and the latency to peak (D) in response to TG in control (filled circles) and H₂O₂ (open squares) treated cells. A total of 118 (control) and 168 (H₂O₂) cells were analysed from 3–4 experiments for each experimental condition. C and D, the TG-induced peak ratio and latency to peak of H₂O₂-treated cells was significantly different from untreated controls; P < 0.001.