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Detection of terminal free GlcNAc residues in human platelets using the mutant enzyme M344H-Y289L-β4Gal-T1. Terminal free GlcNAc residues are very rare on human cell surfaces, but they are known to be present in platelets. Platelets express GPIb — glycoprotein Ib– which contains b-GlcNAc-terminating immature glycans. Upon short-term cooling, these receptors cluster at 4 °C (20).
