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. Author manuscript; available in PMC: 2014 Jan 16.
Published in final edited form as: Bioconjug Chem. 2013 Jan 3;24(1):144–152. doi: 10.1021/bc300542z

Figure 7.

Figure 7

O-GlcNAc detection using the double mutant enzyme M344H-Y289L-β4Gal-T1. UDP-keto-galactose was transferred to a-crystallin protein using the Y289L-M344H mutant. A: Detection of the coupled biotin was performed using streptavidin conjugated with HRP and detected by Western blot analysis (lane 5). B,: Coomasie Blue staining. Lane 1: positive control using Y289L; lane 2: negative control where UDP-2-keto-Gal was omitted from transfer reaction; lane 3: Y289LM344H transfers UDP-2-keto Gal to alpha crystallin.