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. 2012 Oct 22;4(1):161–170. doi: 10.1021/cn300146w

Figure 6.

Figure 6

Comparison of lymphocyte versus platelet uptake of IDT307 in mixed blood cell populations by flow cytometry. Peripheral blood cell samples isolated from humans or rhesus monkeys containing both platelets and lymphocytes were analyzed for IDT307 uptake by flow cytometry. (A) SERT-specific function is not detectable in human lymphocytes using the SERT inhibitors paroxetine (PRX; 100 nM), S-CIT (100 nM), clomipramine (CMI; 100 nM), or 5-HT (500 μM). A high concentration of imipramine (IMI; 100 μM) caused a modest decrease in IDT307 uptake in human lymphocytes. (B) By contrast, human platelet SERT function is almost completely abolished in the presence of the same concentrations of inhibitors used in human lymphocytes. (C) IDT307-associated fluorescence (green bar) in rhesus lymphocytes is not inhibited by different concentrations of S-citalopram (S-CIT) or serotonin (5-HT; 500 μM). (D) Similar to human platelets, rhesus platelet IDT307 uptake is significantly inhibited by S-CIT or 5-HT. **P < 0.01 and ***P < 0.001 versus control. N = 3–6 samples per condition.