Skip to main content
. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: Neuron. 2013 Jan 9;77(1):83–98. doi: 10.1016/j.neuron.2012.11.035

Figure 1.

Figure 1

Zfhx1b expression in the MGE is required for interneuron migration at E12.5. (A-C) Zfhx1b RNA expression detected by in situ hybridization in control and conditional Zfhx1b mutant telencephalons. (B) Nkx2.1-Cre. (C) DlxI12b-Cre. Black arrowhead in B shows loss of Zfhx1b expression in the MGE VZ (except dorsal-most MGE). White arrowheads in B and C show loss of Zfhx1b expression in the SVZ of the MGE. X in B shows loss of Zfhx1b expression in the SVZ/MZ of the LGE. (D-P’) Coronal hemisections of the telencephalon comparing gene expression in three rostral-to-caudal planes of section in control (left side) and Zfhx1b Nkx2.1-Cre conditional mutants (right side). (D-F’) Two color immunofluoresence detection of EGFP (green) and NKX2-1 (red). (G,G’) Higher magnification view of two color immunofluoresence detection of EGFP (green) and Nkx2-1 (red) in control (G) and Zfhx1b mutant (G’). Solid white arrowheads show increased numbers of cells that express both EGFP (green) and NKX2-1 (red) in the mutant's LGE/Str. In the wild type cortex, black arrowheads (with white outline) show that cells express EGFP (green) and not NKX2-1. (H-P’) In situ hybridization expression analysis at E12.5 of Nkx2-1 (H-J’) and Lhx6 (K-M’), and at E13.5 of Nkx2-1 (N-P’). Asterisks in (N-P’) show increased numbers of labeled cells in striatum. X in panels (K’-M’) notes the loss of labeled cells in the cortex. Abbreviations: Cx: cortex; e: ectopia in region of the ventral striatum and central nucleus of the amygdala; GP: globus pallidus; LGE: lateral ganglionic eminence; MGE: medial ganglionic eminence; MZ: mantle zone; Str: Striatum; SVZ: subventricular zone; VPd: ventral pallidum; VZ: ventricular zone. Scale bars equal 500μm (A and D), and 300 μm (G).