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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cancer Discov. 2012 Dec 14;3(1):82–95. doi: 10.1158/2159-8290.CD-12-0404

Figure 2. B-cell precursors in the spleen of Eμ-Myc mice are reduced by mTORC1 inhibition.

Figure 2

6–7 week old wild-type littermate control mice (WT) (n=5), Eμ-Myc mice treated with placebo (P) for 2 weeks (n=5) and Eμ-Myc mice treated with everolimus (E) for 2 weeks (n=7) were analyzed. (A) Average spleen weight (P vs E, p=0.001). (B) Percent B220+ splenocytes (P vs E, p=0.001). (C) May Grunwald-Giemsa stained cytospin preparations of unsorted splenocytes (top) and purified B220+ cells (bottom) in a representative mouse from each group. (D) FACS plots showing surface expression of IgM and IgD in B220+ gated splenocytes for a representative mouse from each group. Error bars represent the SEM. p values were generated using a Student’s unpaired 2-tailed t-test, *=p<0.05.